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Expression is enhanced by the presence of a CAG promoter. These Ai40D mice are useful for optogenetic studies to express an inhibitory opsin that effectively silences the activity of cortical neurons and perhaps other excitable cell types such as muscle cells and immune cells. Fusion protein expression following exposure to cre can be detected by native EGFP fluorescence and in situ hybridization using an EGFP probe [and presumably by antibody staining immunohistochemistry ; although this was not tested by the donating investigator]. The donating investigator did not examine ArchT-EGFP fusion protein expression in tissues other than brain, and did not attempt to generate homozygous mice to date January For characterization information, see images at the Allen Institute for Brain Science website Ai40 images. The bacterial opsins are retinal-binding proteins that combine a light-sensitive domain with an ion channel or pump; providing light-dependent ion transport, membrane potential alteration, and sensory functions to bacteria. Unlike light-driven chloride pumps that enter long-lasting inactive states in response to light, Arch spontaneously recovers from light-dependent inactivation.
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